Fig. 5: triDTCs and harzianol I (1) regulate Trichoderma chlamydospore and A. oryzae sclerotia formation. | Nature Communications

Fig. 5: triDTCs and harzianol I (1) regulate Trichoderma chlamydospore and A. oryzae sclerotia formation.

From: Noncanonical terpene cyclases for the biosynthesis of diterpenoids regulating chlamydospore formation in plant-associated Trichoderma

Fig. 5: triDTCs and harzianol I (1) regulate Trichoderma chlamydospore and A. oryzae sclerotia formation.

a Colony growth comparison between wild-type (WT) and Δtri4155 T. atroviride B7 strains after 2 days of culture. The growth radius is represented as mean radius ± SD (n = 3 biological replicates. Statistical analysis was performed using t-test). b Antagonism assays of T. atroviride B7 against F. graminearum after 6 days: (i) WT T. atroviride (bottom) vs. F. graminearum (top); (ii) Δtri4155 mutant (bottom) vs. F. graminearum (top); (iii) self-pairing control of F. graminearum. c Fungal phenotypes after 7 days of culture: (i) WT showing normal hyphae and chlamydospores; (ii) Δtri4155 with aberrant hyphae and lacking chlamydospores; (iii) Δtri4155 supplemented with 10 μM compound 1 showing conidiation; (iv) Δtri4155 supplemented with 400 μM compound 1 restoring chlamydospore formation. scale bar = 50 μm. Each experiment contains more than 3 independent repeats. d Chlamydospore formation in T. asperellum and T. asperellum-ΔtriDTC (1-day culture), as well as in T. gamsii and T. gamsii-ΔtriDTC (3-day cultures). scale bar = 50 μm. Each experiment contains 3 independent repeats. e Sclerotia formation in A. oryzae NSAR1 (control), A. oryzae-tri4155 (heterologous expression of tri4155 gene), and A. oryzae treated with compound 1 (A. oryzae-S1) or 2 (A. oryzae-S2). Source data are provided as a Source Data file.

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