Fig. 5: Comparison of data-dependent acquisition (DDA) and data-independent acquisition (DIA) methods using the EVGpro method and evaluation of EVGpro in ultra-low amounts of biofluids. | Nature Communications

Fig. 5: Comparison of data-dependent acquisition (DDA) and data-independent acquisition (DIA) methods using the EVGpro method and evaluation of EVGpro in ultra-low amounts of biofluids.

From: A functionally tunable magnetic nanochains platform for N-glycoproteomic analysis of extracellular vesicles from ultratrace biofluids

Fig. 5: Comparison of data-dependent acquisition (DDA) and data-independent acquisition (DIA) methods using the EVGpro method and evaluation of EVGpro in ultra-low amounts of biofluids.

A, B The number of identified N-glycoproteins (A) (means ± SD, n = 3 independent experiments) and their overlap (B) in seminal plasma EV using DDA (pink), direct-DIA (blue), and library-DIA (green). C, D The number of proteins (C) and N-glycopeptides (D) identified by EVGpro from 1 μL seminal plasma using library-DIA method based on magnetic stirrer (blue) and 3D-shaking rotator (pink), respectively (means ± SD, n = 3 independent experiments). E, F The number of proteins (E) and N-glycopeptides (F) identified by EVGpro from 1 μL tear using the DIA method (means ± SD, n = 3 independent experiments). DDA, data-dependent acquisition; DIA, data-independent acquisition. Source data are provided as a Source Data file.

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