Fig. 1: Characterization of 6OMTs and NMTs in H. cordata. | Nature Communications

Fig. 1: Characterization of 6OMTs and NMTs in H. cordata.

From: Gene duplication and clustering underlie the conservation and diversification of benzylisoquinoline alkaloid biosynthesis in plants

Fig. 1

a Images of H. cordata showing its leaves and rhizomes. b Detection of four major 1-BIAs (compared to standards) in plant tissues of H. cordata using UPLC-MS/MS. Extracted ion chromatograms are shown. c Enzyme assay of Hc6OMT1 or Hc6OMT2 with supply of norcoclaurine (NCC) in transient N. benthamiana system. Coclaurine (COC) was exclusively detected in samples expressing 6OMTs by UPLC-MS, compared to a chemical standard. d Enzyme assay of HcNMTs with supply of COC in transient N. benthamiana system. Aligned extracted ion chromatograms are shown for the standards and tobacco samples in UPLC-MS analysis. Mono- or di-methylated products are shown. In both (c, d), tobacco leaves expressing with a MNG fluorescence protein were used as negative control. e Partial sequence alignment for HcNMTs and characterized CNMTs. A (S)-reticuline (RTC) N-methyltransferase PsRNMT (Papaver somniferum; Ranunculales) with di-methylation functionality is included. The key amino acid residue is highlighted in a red box. f Proposed MGC biosynthetic pathways in H. cordata.

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