Fig. 4: Mis-regulation of alternative splicing causes functional defects in SAH liver proteome.
From: Dysregulated RNA splicing impairs regeneration in alcohol-associated liver disease
a Distribution of exons misregulated in SAH patients across gene body and their breakup into transcript regions. b Effect of misregulated exons on ORF of encoded transcript. c Pie chart showing the fraction of alternative spliced genes that encode proteins with known secreted isoforms. d Venn diagram showing the intersection of alternative spliced genes in SAH patients with known secreted and membrane-bound isoforms. e Heatmap showing numbers of predicted alternative splicing induced functional defects in protein products from SAH and AC patients. f RT-PCR based splice assays validating alternative splicing changes in SAH patients. RT-PCR assays for alternative exons were repeated atleast twice and representative results are presented. SAH severe alcohol-associated hepatitis, AC alcohol-associated cirrhosis.
