Fig. 4: AmbH is an iterative module for polyketide backbone assembly in ambruticin biosynthesis. | Nature Communications

Fig. 4: AmbH is an iterative module for polyketide backbone assembly in ambruticin biosynthesis.

From: Genetic engineering of Sorangium cellulosum reveals hidden enzymology in myxobacterial natural product biosynthesis

Fig. 4: AmbH is an iterative module for polyketide backbone assembly in ambruticin biosynthesis.

a Previous in vitro studies of AmbG by Hahn and co-workers37. b HPLC trace of the So ce10 ΔambG mutant. c Structural elucidation of ambruticin G (6). d Feeding study of ambruticin G (6) by the ΔambA-I/ΔambJ mutant. e Feeding study of ambruticin G (6) by the ΔambA-I mutant. f The iterative module AmbH converts ambruticin G to ambruticin J, which is further metabolised by AmbJ and AmbK along with downstream enzymes to the final ambruticin VS compounds.

Back to article page