Fig. 1: Crystallographic fragment screening on co-expressed ZIKV NS2B-NS3 protease. | Nature Communications

Fig. 1: Crystallographic fragment screening on co-expressed ZIKV NS2B-NS3 protease.

From: Combined crystallographic fragment screening and deep mutational scanning enable discovery of Zika virus NS2B-NS3 protease inhibitors

Fig. 1

a Domain boundaries of constructs gZiPro, bZiPro and cZiPro. b Structure superimposition of bZiPro structure 5GPI (NS2B coloured in cyan, NS3 coloured in purple) and cZiPro structure 8PN6 (NS2B coloured in yellow, NS3 coloured in grey) with an RMSD of 0.2 Å. c Enzymatic activities of protease constructs cZiPro, bZiPro and gZiPro. Activities were measured using a fluorescence-dose-response assay with Bz-nKRR-AMC as substrate. Data points represent mean values, and error bars indicate standard deviations (SD) from three independent replicates (n = 3). d Surface view of ZIKV NS2B-NS3 fragment screening output in two orientations. The NS3 protein surface is coloured in grey and NS2B is coloured in yellow. Identified fragments are shown as sticks. Source data are provided as a Source Data file.

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