Fig. 3: Deep mutational scanning to measure mutational tolerance of ZIKV NS2B-NS3. | Nature Communications

Fig. 3: Deep mutational scanning to measure mutational tolerance of ZIKV NS2B-NS3.

From: Combined crystallographic fragment screening and deep mutational scanning enable discovery of Zika virus NS2B-NS3 protease inhibitors

Fig. 3

a The heatmap of NS2B-NS3 residues indicating the mutational effect of each amino acid substitutions in the ZIKV NS2B-NS3 protease. Blue mutations are deleterious for viral growth in Huh-7.5 cells relative to wildtype, white mutations are neutral, and red mutations increase growth in Huh-7.5 cells. Wildtype amino-acid identities at each site are denoted by an ‘X’. In general, most mutations decrease the fitness of the virus in Huh-7.5 cells. b Distribution of fitness estimates for all mutations. The distributions of fitness effects of stop-codon mutants, Catalytic triad mutants and the remaining mutants are coloured in blue, orange and green, respectively. The black vertical line indicates the threshold −1.0 (Log2 (DMS effect)) for fitness. c Fitness view of ZIKV NS2B-NS3 protease. Mutational tolerance is mapped onto the structure of ZIKV NS2B-NS3 (PDB ID: 8PN6). Residues marked in white do not tolerate changes to that site, while residues marked in red tolerate a range of changes. The number of changes tolerated is indicated by the number and the colour. Source data are provided as a Source Data file.

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