Fig. 5: CFAP91 immunoprecipitates with multiple RS3 proteins.
From: Proximity labeling of axonemal protein CFAP91 identifies EFCAB5 that regulates sperm motility
a A volcano plot of the results from IP-MS studies using an anti-FLAG antibody. When comparing Cfap91-/- TG males to WT males, proteins with fold change >2 and P < 0.05 are considered as significantly upregulated, and dots are color-coded in magenta. An unpaired two-tailed t-test was performed for statistical analysis. b Immunoblotting was performed after IP with an anti-FLAG antibody. Signals of CFAP251, LRRC23, IFT140, and BBS2 were found in Cfap91-/- TG but not WT. IZUMO1 served as a loading control of inputs. c Immunoblotting was performed after IP with an anti-LRRC23 antibody or rat IgG, on Cfap91-/- TG testicular lysate. A band of FLAG was found in the IP product using the anti-LRRC23 antibody. d Immunoblotting was performed after IP using P15 testes from WT and Cfap91-/- TG males. A band of CFAP251 was found in Cfap91-/- TG but not WT, while bands of LRRC23 were not found in either Cfap91-/- TG or WT. Acetylated tubulin (Ac-TUB) served as a loading control for input lysates. e Immunohistochemistry of Cfap91+/- and Cfap91-/- testicular sections. LRRC23 and CFAP251 were co-localized with Ac-TUB, while this co-localization was not found in Cfap91-/- testicular sections. f Immunoblot analyses of testes and cauda epididymal spermatozoa from WT and Cfap91-/- males. Alpha-tubulin (α-TUB) served as a loading control.
