Fig. 4: MOP-1 J1 mESC possesses differentiation potential.

A, B UMAP projection of EB of the atlas data set. In the plot, cells are colored by lineage assignment, and colored by partitioning into 8 clusters or different treatment EB (–LIF EB and –MOP-1 EB). Cells from –LIF EB are blue, cells from –MOP-1 EB are red (B). C Prop Plots showing the proportion of three germ layers for –LIF and –MOP-1 EB differentiation. D Visualization of RNA velocities on the UMAP projection with information for the 8 clusters of –LIF EB and –MOP-1 EB. E Expression of endoderm (Fst, Kdr and Sox17), mesoderm (Hand1) and ectoderm markers (Neto2) in –MOP-1 EB and –LIF EB. F Violin plot of the score of apoptosis in –LIF EB and –MOP-1 EB. G Hematoxylin and eosin (H&E) stained cross-sections of –LIF EB and –MOP-1 EB. Scale bar, 20 μm. H Quantitative analysis of the ratio of dead cells area (left panel, mean ± s.e.m, n = 8 from 4 mice teratoma samples per group) and number of dead cells (right panel, mean ± s.e.m, n = 9 from 4 mice teratoma samples per group) of H&E stained cross-sections of –LIF EB and –MOP-1 EB. Data in (F) and (H) are analyzed by a two-tailed unpaired t-test. *P  <  0.05, ****P  <  0.0001, –LIF EB vs. –MOP-1 EB.