Fig. 2: RNAseq analysis of Arabidopsis Col-0, d14, kai2 and max2 roots upon zaxinone treatment. | Nature Communications

Fig. 2: RNAseq analysis of Arabidopsis Col-0, d14, kai2 and max2 roots upon zaxinone treatment.

From: Arabidopsis response to the apocarotenoid zaxinone involves interference with strigolactone signaling via binding to DWARF14

Fig. 2: RNAseq analysis of Arabidopsis Col-0, d14, kai2 and max2 roots upon zaxinone treatment.The alternative text for this image may have been generated using AI.

a Plant phenotypes of 5-weeks old Arabidopsis Col-0 and mutants grown in hydroponic media. b Venn diagrams showing zaxinone (Zax) responsive genes (DEGs) measured by RNAseq in Atd14, Atkai2, and Atmax2 mutant backgrounds in response to zaxinone (20 µM). The expression of 545, 697 and 786 DEGs (up and down) was modulated by zaxinone in the wild type, however the expression of these DEGs remained unchanged in the Atd14, Atkai2 and Atmax2 mutant backgrounds, respectively. c Heatmap representation showing the expression patterns of genes involved in ABA and SL biosynthesis in Col-0, Atd14, Atkai2 and Atmax2 mutant backgrounds obtained from the RNAseq data (DESeq2 R package was used to calculate p-values which were adjusted with Benjamini and Hochberg’s tests; specific padj-values for the expression of NCED2, NCED3, CCD7 and CCD8 are provided in the Supplementary Data 1, 11, 13, 15 for Col-0, Atd14, Atkai2 and Atmax2, respectively). All selected DEGs fulfill the threshold Log2 F.C. > 1 (up) or Log2 F.C.< −1 (down) and padj< 0.05 (represented with an * in 2c). The RNAseq experiment was performed by applying 20 µM zaxinone to the hydroponic media of 5-weeks old Arabidopsis plants for 6 hours (n = 4 independent plants).

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