Fig. 5: Substrate recognition mechanism of subfamily Ⅳ. | Nature Communications

Fig. 5: Substrate recognition mechanism of subfamily Ⅳ.

From: Structure-function relationship of the GH168 fucanase reveals an unusual enzyme recognition mechanism for sulfated polysaccharide

Fig. 5: Substrate recognition mechanism of subfamily Ⅳ.

a Cleavage site of subfamily Ⅳ towards different sulfated fucan. b, c MS/MS spectrum of the dominant oligosaccharide in the product of Ta-FUC and Am-FUC by Pb41GH168_Ⅳ, respectively. d Critical residues within the negative subsite region of WfGH168_ Ⅱ. The conserved key residues of the family and the characteristic residues of subfamily Ⅳ are represented by white sticks and red sticks, respectively. e Molecular docking of Pb41GH168_Ⅰ and the ligand F0S-F2,4S-F2S-F2S. The critical residue Q172 is represented by the red stick.

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