Fig. 6: Prominent NE defect occurs under conditions of synthetic lethality.

a Representative images of GFP-BAF with the indicated treatments for 48 h. b Quantification of cells with BAF foci for (a). Data are presented as mean ± SD, n = 3 independent experiments, N > 200 for each experiment. Statistical analysis is performed by two-tailed unpaired Student’s t-test. c Viability assay of RPE-1 cells with the indicated concentrations of ATR-i (VE-821) ± APH treatment for 48 h. Values under different conditions are normalized to the value in DMSO treatment without ATR-i. Data are presented as mean ± SD, n = 9 independent experiments. Statistical analysis is performed by two-way ANOVA. d Representative images of GFP-BAF in WT or BRCA2−/− cells after treatment with DMSO or 5 μM PARP-i (Olaparib) for 48 h. Two BRCA2−/− clones with different genotypes are included. e Quantification of WT or BRCA2−/− cells with BAF foci with indicated treatments. Data are presented as mean ± SD, n = 3 independent experiments, N > 250 for each experiment. Two-way ANOVA is performed for different groups. Two-tailed unpaired Student’s t-test is performed for interested pairs (ns p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001). f Viability assay for WT or BRCA2−/− cells with treatment of the indicated concentrations of PARP-i. Data are presented as mean ± SD, n = 3 independent experiments. Statistical analysis is performed by two-way ANOVA. g Representative images of GFP-BAF in WT or BRCA2−/− cells after treatment of DMSO or 5 μM Polθ-i (ART558) for 48 h. h Quantification of WT or BRCA2−/− cells with BAF foci after treatment with the indicated concentrations of Polθ-i for 48 h. Data are presented as mean ± SD, n = 3 independent experiments, N > 250 for each experiment. Two-way ANOVA is performed for different groups. Two-tailed unpaired Student’s t-test is performed for interested pairs (ns p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001). i Viability assay for WT or BRCA2−/− cells with treatment of the indicated concentrations of Polθ-i. Data are presented as mean ± SD, n = 3 independent experiments. Statistical analysis is performed by two-way ANOVA. For (a, d, g), scale bar = 10 μm. Source data are provided as a Source Data file.