Fig. 2: Screening of ecdysone signaling-mediated TFs necessary for NC dumping.
From: Ecdysone signaling-induced dumpless1 expression controls nurse cell dumping in Drosophila oogenesis
a A Venn diagram representation of TFs that are upregulated in egg chambers during NC dumping (red circle) but downregulated in the ovaries after ecdysone signaling disruption in SFCs (blue circle). b Schematic representation of the structure of a stage 10 egg chamber in Drosophila. c Heatmap showing relatively expressed levels of differentially expressed TFs in different types of cells in an egg chamber at stage 10. d, e Changes in mature egg length after RNAi knockdown of differentially expressed TFs in SFCs driven by c329b-Gal4 (n = 36). Two-tailed t-test was performed. Data are presented as mean values ± SD. f–i’ Confocal medial view images of CG12942 expression (red) in egg chambers of w1118 at stages 9–12, detected by an anti-CG12942 antibody. The yellow arrow indicates newly formed SFCs. j–m’ Confocal images of medial view presenting CG12942-GFP fusion protein (green) in the egg chambers of CG12942::GFP line at stages 9–12. n, o CG12942 expression (red) in the egg chambers at stages 10–11, detected by an anti-CG12942 antibody, in the flip-out Gal4 clones of EcRDN overexpression (green). p EcRE consensus sequence and predicted binding sequence of EcR/USP complex upstream of CG12942. Nuclei are marked by DAPI in cyan. The scale bar represents 50 µm, respectively. SFC stretch follicle cell, MFC mainbody follicle cell, OC oocyte, NC nurse cell, PC polar cell, TFC terminal follicle cell, DAFFC dorsal appendage forming follicle cell, RC ring canal.
