Fig. 4: dumpless1 mutation reduces cortical and cytoplasmic Rho1 enrichment in NCs.

a Analysis of DEGs and PCA of RNA-seq data from the 7-day-old adult ovaries of w¹¹¹⁸ and dumpless1^-/- mutants. b Barplots of GO-enriched biological processes for DEGs associated with Rho1 signaling. c–n’ Confocal images of Rho1 accumulation in NCs at stages 9–12 in w¹¹¹⁸ (c-c’, f-f’, i-i’ and l-l’), dumpless1^-/- (d-d’, g-g’, j-j’ and m-m’) and dumpless1^∆ZAD mutants (e-e’, h-h’, k-k’ and n-n’), stained with an anti-Rho1 antibody. The white and cyan arrows indicate the cortex and cytoplasm of NCs, respectively. F-actin is marked by phalloidin in magenta. The bar represents 50 µm. NC nurse cell, OC oocyte. o Quantified fluorescence intensity of Rho1 in NC cortex at stages 9–12 of w¹¹¹⁸, dumpless1^-/- and dumpless1^∆ZAD mutants. From left to right: n = 4, 6, 4, 11, 11, 15, 16, 15, 16, 15, 16, 16, respectively. p Quantified fluorescence intensity of Rho1 in NC cytoplasm at stages 10–12 of w¹¹¹⁸, dumpless1^-/- and dumpless1^∆ZAD mutants. From left to right: n = 12, 11, 13, 15, 15, 15, 12, 15, 15, respectively. Two-way ANOVA was made for multiple comparisons. Data are presented as mean values ± SD.