Fig. 5: The charge reversal mutation sets the S1 and S2 away from the S4 helix and the pore domain.
From: A physiologically-relevant intermediate state structure of a voltage-gated potassium channel
a-b Top view representations of one VSD and its interactions with the filter helix from the adjacent subunit (FH’). In the inset, the tetrameric structures are shown to facilitate orientation. The FH’ interacting with the VSD is shown in a darker shade of blue for xWT (a), and orange for xE1R/R2E (b). Residues interacting with 221 and 150 are shown as gray and yellow sticks, respectively. Residues that may interact with R227 in the xE1R/R2E structure (b) are shown as orange sticks. The salt-bridge interaction in (a) between E150 and R227 is shown as a black line, polar interactions as blue dashed lines and the potential salt-bridge interaction in (b) as a black dashed line.
