Fig. 2: Oral EVG7 prevents recurrence in a mouse model of rCDI. | Nature Communications

Fig. 2: Oral EVG7 prevents recurrence in a mouse model of rCDI.

From: Experimental glycopeptide antibiotic EVG7 prevents recurrent Clostridioides difficile infection by sparing members of the Lachnospiraceae family

Fig. 2

a Timeline for in vivo model of recurrent C. difficile infection (rCDI) in male C57BL/6 J mice. Following cefoperazone pretreatment, the vancomycin (VAN, n = 8 mice) and EVG7 (n = 12 mice) groups were challenged with C. difficile via oral gavage; the ‘no CDI’ group (n = 4 mice) was not challenged with C. difficile. Treatment with 0.4 mg/mL vancomycin or 0.04 mg/mL EVG7 was administered in the drinking water ad libitum from days 4–9. Mice were monitored for rCDI, which occurred on days 14 and 15 in the VAN group. b Baseline weight loss of mice in ‘no CDI’ (n = 4), VAN (n = 8), and EVG7 (n = 12) groups. Data are mean ± standard deviation. Significance comparing VAN to EVG7 was determined by two-sided mixed-effects model analysis followed by a Tukey’s posttest. c Clinical scoring of mice to monitor severity of CDI in the ‘no CDI’ (n = 4), VAN (n = 8), and EVG7 (n = 12) groups. Clinical score chart provided in Supplementary Table 2. Visual presentation and statistics as described for (b). d Total spores and vegetative cells of C. difficile in colony-forming units (CFUs) per gram of fecal content in the VAN (n = 8) and EVG7 (n = 12) groups. Each dot represents an individual fecal sample, bars represent the mean with error bars showing the standard deviation. Stool was not collected from deceased or very sick animals. Values below LOD were set to 1000. Significance and statistics as described for (b). e Total spores and vegetative cells of C. difficile in CFUs per gram of cecal content on day 14/15 (VAN, n = 6) or day 18 (EVG7, n = 12) (P < 0.0001). Each dot represents an individual cecal sample, bars represent the mean with error bars showing the standard deviation. Values below LOD were set to 1000. Significance was determined by a two-sided Mann-Whitney test. f Toxin activity in cecal content as determined by Vero cell cytotoxicity assay (P < 0.0001). Visual presentation and statistics as described for (e). The dashed line represents the limit of detection for the toxin activity (reciprocal log titer). For all statistical tests: *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001. Graphs were generated in GraphPad Prism 10 and further compiled in Adobe Illustrator 2024. Source data are provided as a Source Data file.

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