Fig. 3: Oral EVG7 spares the gut microbiota in a mouse model of rCDI.
Microbiome analysis of DNA extracted from cecal content samples (a–e)) of 0.4 mg/mL vancomycin-treated (VAN) mice (on day 14/15, n = 6 mice) and 0.04 mg/mL EVG7-treated mice (on day 18, n = 12 mice) or from fecal samples collected on various days (f, g)). Data accompanying Fig. 2. a Principal Coordinates Analysis (PCoA) plot based on Aitchison’s distances between cecal content samples. The variance contribution of the first and second principal coordinates is shown on the x- and y-axis, respectively. Each point represents the microbial community of an individual sample. Ellipses correspond to 95% confidence regions. The significance of the clustering (P = 0.001) was calculated with permutational multivariate analysis of variance (PERMANOVA). b Shannon diversity in cecal content (P = 0.0001). Significance was determined by a two-sided Mann-Whitney test. Box plots show the median (center line), the first and third quartiles (bounds of the box). Whiskers to show the 1.5 of the IQR. Asterisks indicate statistical significance: ***P ≤ 0.001; ****P ≤ 0.0001. Each dot represents an individual cecum sample. c Chao1 richness in cecal content (P = 0.0009). Visual presentation and statistics as described for (b). d Relative abundance of the C. difficile ASV in cecal content (P = 0.0001). Visual presentation and statistics as described for (b). e Heatmap representing the core ASVs detected in the cecal content. The core is defined by a 0.01 detection and a 0.3 prevalence threshold. The heatmap color represents centered log-ratio (CLR) transformed ASV relative abundances. The bar plot insert depicts the prevalence of the ASV. Treatments are clustered based on hierarchical clustering using Euclidean distance. f Principal response curve showing time-dependent antibiotic treatment effects in feces of VAN (reference line) and EVG7 groups. Absolute abundances were log10 transformed. Taxa with bk values above 0.3 or below −0.3 are displayed. g Longitudinal relative abundance of Lachnospiraceae family members in feces. Each point represents an individual stool sample. The measure of center of the regression line is fitted with a smooth local regression (LOESS), error bands indicate a 95% confidence interval. Graphs were generated in R99 v4.1.2 and further compiled in Adobe Illustrator 2024. Source data are provided as a Source Data file.
