Fig. 2: Munc13-4 deficiency in tumor cells enhances T cell infiltration and activation.

Flow cytometric quantification of the percentage of CD45⁺CD3⁺CD4⁺ and CD45⁺CD3⁺CD8⁺ T cells among total cells in the tumors (A), spleens (B), and draining lymph nodes (C) of BALB/c mice (n = 5 mice), 21 days after mammary gland injection with 3 × 10⁵ control or Munc13-4 KO 4T1 cells per mouse. Quantification of the percentage of granzyme B⁺ (GzmB⁺) (D), Ki67⁺ (E), and IFNγ⁺ (F) cells among CD45⁺CD3⁺CD4⁺ and CD45⁺CD3⁺CD8⁺ T cells within tumors from orthotopic mouse models of breast cancer generated by control or Munc13-4 KO 4T1 cells (n = 5 mice). Quantification of the percentage of granzyme B⁺ (G), Ki67⁺ (H), and IFNγ⁺ (I) cells among CD45⁺CD3⁺CD4⁺ and CD45⁺CD3⁺CD8⁺ T cells within spleens from orthotopic mouse models of breast cancer generated by control or Munc13-4 KO 4T1 cells (n = 5 mice). Quantification of the percentage of granzyme B⁺ (J), Ki67⁺ (K), and IFNγ⁺ (L) cells among CD45⁺CD3⁺CD4⁺ and CD45⁺CD3⁺CD8⁺ T cells within the draining lymph nodes from orthotopic mouse models of breast cancer generated by control or Munc13-4 KO 4T1 cells (n = 5 mice). Box plots show the median (center line), interquartile range (box), minima and maxima (whiskers), and all individual data points (dots). All p-values were calculated by two-tailed Multiple t-tests. Source data are provided as a Source data file.