Fig. 5: αGD2 DCP-IL-12/EVIR do not induce liver or kidney toxicity in mice.

a Procedure to study liver and kidney toxicity following systemic DCP-IL-12/EVIR inoculation in tumour-free male and female mice. The experiment was performed once. b Serum creatinine at day 4 post-DCP injection (mean ± s.e.m.; n = 5 male or female mice). c Serum AST and ALT at day 4 post-DCP injection (mean ± s.e.m.; n = 5 male or female mice, except for MCS and MCD, n = 6 male mice). The mice in the MCS and MCD groups originated from an independent experiment (separated by a dotted line). Statistical analysis by two-tailed Mann-Whitney test comparing mice with same sex. d Serum AST and ALT at day 4 and 36 post-injection (mean ± s.e.m.; n = 5 male or female mice). Statistical analysis by repeated measures two-way ANOVA with Sidak’s correction for multiple comparisons (comparing mice with same sex). e Representative H&E-stained kidney sections of female mice from the indicated treatment groups. Scale bar: 0.1 mm. f, Representative H&E-stained liver sections of male or female mice from the indicated treatment groups. Arrows indicate areas of mild inflammatory infiltration in the liver. Scale bar: 0.1 mm. g Frequency of male or female mice (n = 5) exhibiting histological features of inflammation, steatosis, ballooning, or fibrosis in liver sections. Each data point in all panels represents one sample from an independent mouse except in (g), in which values represent the percentage of mice per group showing the indicated pathology grade. Source data are provided as a Source Data file.