Fig. 6: Human DCP-IL-12/EVIR promote antigen internalization and presentation. | Nature Communications

Fig. 6: Human DCP-IL-12/EVIR promote antigen internalization and presentation.

From: Dendritic cell progenitors engineered to express extracellular-vesicle–internalizing receptors enhance cancer immunotherapy in mouse models

Fig. 6: Human DCP-IL-12/EVIR promote antigen internalization and presentation.

a Procedure to produce and differentiate engineered human DCP progeny from CD34+ cord-blood cells. b, c Representative dot plots showing the expression of CtrlR and EVIR in human DCP progeny and moDCs. d Schematic representation of the assay used to study EV internalization by transduced human DCP progeny and moDCs. PKH26 mean fluorescence intensity (MFI) in transduced human DCP progeny (e) or human moDCs (f) measured by flow cytometry from two independent donors and two biological replicates for each (mean ± s.e.m.). g Schematic representation of the assay used to study antigen cross-dressing by transduced human DCP progeny or human moDCs. Antigen presentation by human DCP progeny (h) or human moDCs (i) engineered to express CtrlR, EVIR or hIL-12/EVIR (mean ± s.e.m.; DCP-CtrlR, n = 3; EVIR and hIL-12/EVIR, n = 2; moDC-CtrlR, n = 3; EVIR and hIL-12/EVIR, n = 2 biological replicates from a single donor).Each data point in all panels represents biological replicates from one or two independent donors. Source data are provided as a Source Data file.

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