Fig. 2: Eosinophils decrease the fungal burden and improve survival in a murine model of systemic candidiasis.
A Diagram illustrating the systemic candidiasis model to assess the fungal burden (5 × 105 yeast/mouse). Created in BioRender. Zaffran, I. (2025) https://BioRender.com/9xkicks. B C. albicans CFU in the kidneys, liver, and spleen, of WT and Δdbl/GATA mice 2 days post-infection (dpi); n = 10 (WT) and 11 (Δdbl/GATA) mice/group for kidneys and liver samples and n = 9 for spleen samples, all collected from 3 independent experiments. C, D IL-6 (n = 7 for WT; n = 11 for Δdbl/GATA mice) and IFN-γ (n = 6) levels in serum (C) and IFN-γ levels in kidneys (D; n = 11 for WT; n = 14 for Δdbl/GATA mice) of C. albicans-infected WT and Δdbl/GATA mice. E Diagram illustrating the “survival” systemic candidiasis model (5 × 104 cells/mouse). Created in BioRender. Zaffran, I. (2025) https://BioRender.com/9xkicks. F Survival rate (percent survival) of WT (black curve) and Δdbl/GATA (red curve) mice; n = 12 mice/group in 2 independent experiments. G IFN-γ levels produced by human eosinophils culture in medium or with C. albican at 0.1 and 1 MOI for 3 h; n = 2 biological replicates. Data show the mean ± SEM (B–D) or mean ± SD (G). Significance was analyzed by two-tailed student t test (B–D and G), survival curve was analyzed by Log-rank (Mantel Cox) test; *P ≤ 0.05; **P ≤ 0.01.
