Fig. 7: Recombinant CCL28 protein ameliorates revascularization disorder in diabetic mice. | Nature Communications

Fig. 7: Recombinant CCL28 protein ameliorates revascularization disorder in diabetic mice.

From: CCL28 contributes to angiogenesis and cardiac repair through CCR10+ endothelial cells after myocardial infarction in male mice

Fig. 7

A–H the hindlimb ischemia (HI) and myocardial ischemia (MI) were constructed on wild-type and db/db mice. A Representative bands of CCL28 and ACTB protein in the gastrocnemius of mice subjected to sham or HI surgery at 7 days was measured by Western blot assay (biological replicates, n = 3 per group). B Representative bands of CCL28 and ACTB protein in the hearts of mice subjected to sham or MI surgery at 3 days measured by Western blot assay (biological replicates, n = 3 per group). C Representative bands of CCR10 and ACTB protein in the gastrocnemius of mice subjected to sham or HI surgery at 7 days measured by Western blot assay (biological replicates, n = 3 per group). D Representative bands of CCR10 and ACTB protein in the hearts of mice subjected to sham or MI surgery at 3 days measured by Western blot assay (biological replicates, n = 3 per group). E–H Flow cytometry-based representative dot plots and quantification of CCR10+CD31+ endothelial cells isolated from the gastrocnemius of mice subjected to sham or HI surgery at 7 days, and the hearts of mice subjected to sham or MI surgery at 3 days (biological replicates, n = 4 per group, Unpaired two-tailed Student’s t-test). I–M the hindlimb ischemia (HI) and myocardial ischemia (MI) were constructed on db/db mice treated with mouse recombinant CCL28 protein (rCCL28, 50 μg/kg, every four days) or vehicle. I–L Representative immunofluorescence images and quantification of vessel numbers in the Vehicle and rCCL28 groups of gastrocnemius 7 days post HI, and hearts 3 days post MI with staining for CD31 (red) and nuclei (blue) (biological replicates, n = 5 per group, Unpaired two-tailed Student’s t-test). Scale bar, 200 μm. M Representative images and quantification of blood flow in mice examined by laser Doppler imaging at specific time points (biological replicates, n = 8 per group, Two-Way ANOVA using Tukey multiple comparisons test). Data are expressed as mean ± SD. Source data are provided as a Source Data file.

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