Fig. 2: Proteomic characterization of 1D4-immunopurified TRPML1-mNeonGreen-1D4 and TMEM192-mCherry-1D4 organelles.
From: Visualization of lysosomal membrane proteins by cryo electron tomography
a Immunoblotting for protein markers of several subcellular compartments measured in the post-nuclear supernatant (PNS) and 1D4 immunopurified (1D4 IP) lysosomes of lysates prepared from HEK 293 cells stably overexpressing TRPML1-mNeonGreen-1D4 or TRPML1, as a control and (b) TMEM192-mCherry-1D4 or TMEM192-3xHA, as a control. Experiment was performed one time. Source data are provided as a Source Data file. c Volcano plot of fold-enrichment of proteins identified between control (TRPML1) and TRPML1-mNeonGreen-1D4 triplicate 1D4 immunopurifications and (d) control (TMEM192-3xHA) and TMEM192-mCherry-1D4 triplicate 1D4 immunopurifications. Dashed lines indicate threshold of Log2FC > 1.0 and < -1.0 and a p-value < 0.05. The green and red dots indicate proteins associated with the endosome (GO:0005768) and lysosome (GO:0005764). Volcano plots were generated using a two-tailed t-test without any p-adjustment methods. Source data are provided as a Source Data file.
