Fig. 4: Organelle morphology of immunopurified and eluted TRPML1-mNeonGreen-1D4- and TMEM192-mCherry-1D4-positive lysosomes and their associated membrane proteins.
From: Visualization of lysosomal membrane proteins by cryo electron tomography
a 2D tomographic slices of organelles immunopurified and eluted using TRPML1-mNeonGreen-1D4 as the target (top) (n = 617) and their corresponding 3D reconstructions after segmentation (bottom). Membranes are shown in green. b 2D tomographic slices of organelles immunopurified and eluted using TMEM192-mCherry-1D4 as the target (top) (n = 55) along with their segmented 3D reconstructions (bottom), where membranes are shown in slate blue. Arrowheads indicate V-ATPase (purple), Flotillin (orange), and Clathrin (gold). Scale bar is 50 nm.
