Fig. 5: Sub-tomogram averaging of lysosomal membrane and membrane-associated proteins of immunopurified and eluted TRPML1-mNeonGreen-1D4-positive lysosomes.
From: Visualization of lysosomal membrane proteins by cryo electron tomography
a Examples of V-ATPase (n = 1325), (c) Flotillin (n = 2927), and (e) Clathrin particles (n = 2024) used for sub-tomogram averaging. Scale bar is 20 nm. (b) Density maps of V-ATPase, (d) Flotillin, and (f) Clathrin obtained from sub-tomogram averaging, overlaid with their respective coordinate models: V-ATPase (PDB: 9BRT), Flotillin (PDB: 9BQ2), and Clathrin (PDB: 1XI4).
