Fig. 2: Characterization of CRISPR/Cas9-mediated GhDP1 mutants (CR-Ghdp1). | Nature Communications

Fig. 2: Characterization of CRISPR/Cas9-mediated GhDP1 mutants (CR-Ghdp1).

From: A dirigent protein redirects extracellular terpenoid metabolism for defense against biotic challenges

Fig. 2

a Schematic diagram of the GhDP1_A1 (left) and GhDP1_A2 (right) gene structures. Pink rectangles denote the positions of single-guide RNA (sgRNA) target sites within the coding regions. The sequences of the sgRNA target sites are presented for both wild-type (WT) plants and the corresponding mutant alleles. b Statistical analysis of the content levels of 3, 4, and 5a ~ 5d in various organs of GhDP1-knockout plants (CR-Ghdp1 #1/2), in comparison to wild-type (WT) plants (mean ± s.d., n = 3 biological replicates). P values were determined by a two-tailed unpaired t-test. Source data are provided as a Source Data file.

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