Fig. 2: Bioenergetically heterogeneous BMDMs harbor redox-diverse bacteria.
From: Bioenergetic reprogramming of macrophages reduces drug tolerance in Mycobacterium tuberculosis
A Workflow of the flow-sorting coupled Seahorse Extracellular Flux analysis; B, C A modified mitostress test was performed to calculate mitochondrial parameters. BR- basal respiration, ATP- ATP production, H + -leak- proton leak; D, E ECAR test was performed to assess the parameters associated with glycolysis in the three sorted BMDM subpopulations. Gly- glycolysis, GC- glycolytic capacity, NGA- non-glycolytic acidification; F Mitochondrial ROS in Mtb-roGFP2 infected BMDMs. Antimycin A used as the positive control; G Cellular ROS measured in Mtb-roGFP2 infected BMDMs, 100 µM menadione used as the control. MFI- median fluorescence intensity, a.u.-arbitrary units. Data are expressed as mean ± S.D., representative of three independent experiments. p-value determined using a Student’s two-tailed t-test with Welch’s correction for comparison between two groups or one-way ANOVA with Dunnett’s T3 test for multiple comparisons. Figure 2A: Created in BioRender. Yadav, V. (2025) https://BioRender.com/y42m819. Source data are provided as a Source Data file.
