Fig. 3: Genome-assisted identification of Lr.ace-4A.
From: Genome-assisted identification of wheat leaf rust resistance gene Lr.ace-4A/Lr30
a Susceptible EMS mutants used to isolate the Lr.ace-4A gene. Infection types for PI 192051, seven independent susceptible EMS mutants (m13, m12, m22, m1, m23, m2, and m10), and Rusty inoculated with Pt pathotype PHQS. R resistant, S susceptible. b Integrative Genomics Viewer (IGV) snapshots showing RNA-seq reads from susceptible EMS mutants mapped to the PI192051.r1.4 AG0210600 gene. Blue arrows indicate the positions of EMS-induced point mutations (G/C to A/T) identified in the seven susceptible mutants. WT, wild-type PI 192051. c Gene structure of Lr.ace-4A. The positions of the EMS-induced mutations are indicated by blue arrows. Gray boxes represent untranslated regions, blue boxes indicate coding exons, and gray lines denote introns. The start and stop codons are indicated by black arrows. d Protein structure of Lr.ace-4A. The coiled-coil (CC), nucleotide-binding (NB-ARC), and leucine-rich repeat (LRR) domains are highlighted in yellow, green, and pink, respectively, according to the Pfam protein families database.
