Fig. 4: Volcano plot depicting proteomic data analysis results. | Nature Communications

Fig. 4: Volcano plot depicting proteomic data analysis results.

From: Multifunctionally diverse alkaline phosphatases of Alteromonas drive the phosphorus cycle in the ocean

Fig. 4

The x-axis represents the log-fold change in protein abundance, calculated as the ratio of the average abundance in P-limited cultures to that in control conditions (in biological triplicates). Positive values on the x-axis indicate proteins upregulated under P-limitation, while negative values indicate downregulation. The y-axis displays the negative logarithm (base 10) of the two-sided t-test p-value. Proteins were considered significantly altered if they had a p-value < 0.05 and were at least two-fold upregulated (log2-fold change > 1.0) or downregulated (log2-fold change < −1.0) and are highlighted as dark points. A two-sided Student’s t-test was used to determine significance. Key proteins implicated in phosphate acquisition are highlighted by color: PhoA (green), PhoD (blue), PhoX (purple), and PafA (pink). Among these, PhoD is the only significantly upregulated protein. Among the alkaline phosphatase families, PhoD stands out as the only protein significantly upregulated during P-limitation.

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