Table 2 Data presented as the mean ± standard deviation of curve fitting for cell-specific Vmax turnover rate (Vmax divided by cell abundance), KM Michaelis–Menten constant, and cell-specific Vmax/KM
Enzymatic parameters of Alteromonas mediterranea culture experiments | |||
|---|---|---|---|
Substrate | cell-specific Vmax (mmol cells− 1 s−1) | KM (mmol L−1) | cell-specific Vmax/KM (L cells−1 s−1) |
P-monoesterase | |||
Control | (3.3 ± 1) × 10−17 | (27 ± 0.8) × 10-2 | (1.2 ± 0.1) × 10−15 |
P-limitation | (3.7 ± 0.8) × 10−16 | (3.4 ± 0.3) × 10-3 | (1.1 ± 0.2) × 10−13 |
P-diesterase | |||
Control | (8.1 ± 1.3) × 10−18 | (13.4 ± 5.7) × 10-2 | (6 ± 1.6) × 10-17 |
P-limitation | (1.6 ± 0.4) × 10−16 | (25.4 ± 3.2) × 10-2 | (6.2 ± 1.9) × 10-16 |
P-triesterase | |||
Control | (1.8 ± 1.2) × 10−17 | nd | nd |
P-limitation | (9.8 ± 6.7) × 10−18 | nd | nd |