Fig. 7: ELAVL 1 mediated the effects of MEG3 on mRNA stability of RRAS2.

a Identification of ELAVL 1 as RRAS2 and MEG3 binding protein. b RRAS2 mRNA stability in HASMCs transfected with si-ELAVL 1. n = 3 distinct samples. c Interaction of MEG3 with ELAVL 1 determined by RNA pull-down assay. d Interactions of ELAVL1 with full-length or MEG3 truncations. e Interaction between MEG3 and ELAVL 1 determined by RIP assay. n = 5 distinct samples, two-sided Unpaired t-test with Welch’s correction. f The interaction between RRAS2 and ELAVL 1. n = 5 distinct samples, two-sided Unpaired t-test. g Protein level of R-Ras2 in HASMCs transfected with si-ELAVL 1. n = 6 distinct samples, two-sided Unpaired t-test with Welch’s correction. h Expression of R-Ras2 in HASMCs overexpressing with ELAVL1 and stimulated with Ang II. n = 6 distinct samples for each group. Two-way ANOVA test followed by Turkey’s multiple comparisons test. i HASMCs were overexpressed with MEG3, followed by transfected with si-ELAVL 1 and stimulated with Ang II, the level of RRAS2 were determined. n = 6 independent experiments. Two-way ANOVA test followed by Turkey’s multiple comparisons test. j Schematic of 3′UTR, CDS and 5′UTR of RRAS2 reporter plasmids. k, l Luciferase activity of RRAS2 reporter plasmids. n = 6 independent experiments for each group, two-sided Unpaired t-test. m Schematic prediction of ELAVL 1 binding AU-rich elements (AREs) within the 3′UTR of RRAS2. n Luciferase activity of RRAS2 3′UTR variants (Full, a–e) in HEK293 cells with empty or ELAVL1 overexpressing vector. n = 6 independent experiments for each group, two-sided Unpaired t-test. o RIP analysis of relative enrichment of RRAS2 transcripts in ELAVL 1-immunoprecipitates. n = 5 independent experiments for each group, two-sided Unpaired t-test with Welch’s correction. All data were represented as mean ± SEM. Source data are provided as a Source Data file.