Fig. 4: GNP2 interacts with Gnp4/LAX2.
From: Natural variation of GNP2 enhances grain number to benefit rice yield
a, f Y2H assays showing GNP2 interacts with Gnp4/LAX2 in yeast cells. Yeast cells were cultured on SD minimal medium lacking Trp and Leu (SD/-T-L) or lacking Trp, Leu, His, and Ade (SD/-T-L-H-A). aa, amino acid. b, c RNA in situ hybridization analysis of GNP5 expression during rice inflorescence development. d, e RNA in situ hybridization analysis of GNP2 expression during rice inflorescence development. PBM primary branch meristem, SBM secondary branch meristem. g BiFC assays showing that GNP2 interacts with Gnp4/LAX2 in rice protoplasts. As a negative control, GNP2 and OsGSK3 did not show any interaction. h LCI assays showing that GNP2 interacts with Gnp4/LAX2 in epidermal cells of tobacco leaves. As a negative control, GNP2 and OsGSK3 did not show any interaction. i Co-IP assays showing the interaction between GNP2 and Gnp4/LAX2 in rice protoplasts. j In vitro pull-down assays showing the interaction between GNP2 and Gnp4/LAX2. Scale bars, 200 μm (b–e), 20 μm (g). In a–j, a representative experiment from three independent experiments is shown. Source data are provided as a Source Data file.
