Fig. 2: In vivo protective efficacy of mAbs C34 and C37.

a–d In vivo therapeutic efficacy of C34, C37, and C59 (non-neutralizing control) mAbs against CHIKV infection in mice. a Mice were administered 10 mg/kg of the indicated mAbs by intraperitoneal injection 24 h after a lethal CHIKV challenge. Dpi days post-infection. b Survival rates of each group. The number of mice in each group are indicated in brackets. Survival rates of mAb-treated mice were compared to the PBS group. p = 0.039, 0.0039, and 0.5846 for C34, C37, and C59 groups against the PBS group, respectively. c Percentage of weight change in each group of mice. p = 0.0493 for day1 C34 vs PBS group. d Footpad swelling was measured as the percentage change in footpad size in each group of mice. For day 2, p = 0.0169 for C34. For day 3, p = 0.0002 and 0.0370 for C37 and C59, respectively. For day 4, p = 0.0002 and 0.0001 for C34 and C3,7, respectively. e–h In vivo prophylactic efficacy of C34, C37, and C59 mAbs against CHIKV infection in mice. e Mice were administered 10 mg/kg of the indicated mAbs by intraperitoneal injection 24 h before a lethal CHIKV challenge. f Survival rates of each group, with the number of mice indicated in brackets. Survival rates of mAb-treated mice were compared to the PBS group. p = 0.034, 0.0034, and 0.0528 for C34, C37, and C59 groups against the PBS group, respectively. g Percentage of weight change in each group of mice. For day 3, p = 0.0287 for C34. For day 4, p = 0.0013 and 0.0017 for C34 and C37, respectively. For day 5, p = 0.0215 and 0.0112 for C34 and C37, respectively. For day 6, p = 0.0233 and 0.0088 for C34 and C37, respectively. h Footpad swelling was measured as the percentage change in footpad size in each group of mice. For day 3, p = 0.0294 and 0.0190 for C34 and C37, respectively. For day 4, p = 0.0312 and 0.0478 for C34 and C59, respectively. For day 5, p = 0.0144 for C59. For c, d, g, h, data represent mean ± s.d. of n = 5 biological repeats. For b, f, statistical analysis was performed using the Mantel-Cox log-rank test: **p < 0.01, ***p < 0.001, ****p < 0.0001. For c, d, g, h, statistical analysis was performed using two-way ANOVA and Dunnett’s multiple comparisons test: **p < 0.01, ***p < 0.001, ****p < 0.0001.