Fig. 2: Systemic infection is compromised by deletion of SPI-2 effector cohorts. | Nature Communications

Fig. 2: Systemic infection is compromised by deletion of SPI-2 effector cohorts.

From: Salmonella multimutants enable efficient identification of SPI-2 effector protein function in gut inflammation and systemic colonization

Fig. 2: Systemic infection is compromised by deletion of SPI-2 effector cohorts.

A Experimental scheme to study virulence of S.Tm mutants during systemic infection in vivo. Mice were infected with 103 S.Tm by intraperitoneal injection. Mice were euthanised at day 4 post infection, and bacterial loads in the spleen and liver were quantified by CFU plating to selective media. Created in BioRender. Newson, J. (https://BioRender.com/ngy8g3l). B S.Tm recovered from the liver (left) and spleen (right), (n = 5–7 mice per group). Horizontal bars denote median. Dotted lines denote limit of detection. Statistical differences between WT and indicated groups determined by two-tailed Mann Whitney-U test, (p ≥ 0.05 not significant (ns), p < 0.01 (**), p < 0.001 (***). C Experimental scheme to study relative fitness of S.Tm mutants in vivo by competitive infection. Mice were infected with a mixed inoculum comprising equal volumes of 9 different S.Tm strains each bearing unique chromosomal tags. Mice were euthanised at day 4 post infection. Created in BioRender. Newson, J. (https://BioRender.com/9d0jurp). D Relative proportion of each genetic tag determined by RT-qPCR. Data is presented as the proportion of a given tag relative to the other tags within one animal. Coloured circles represent tagged strain recovered from the liver (left) and spleen (right) of infected mice (n = 5 mice). Horizontal bars denote median.

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