Fig. 1: UBAP1 is recruited to damaged lysosomes and correlates with lysosome recovery.

a Representative image of COS-7 cells expressing GAL3-mbaojin and mYH-UBAP1 without/with LLOMe treatment (1 mM) for 1 h and wash out for indicated time points. b Quantification of GAL3 or UBAP1 positive puncta in (a). M (Mock) n = 10, L (LLOMe) n = 13, 15 min n = 9, 1 h n = 10, 3 h n = 8, 6 h n = 13, 10 h n = 13, 14 h n = 13 cells. c Images and 3D reconstruction of COS-7 cells expressing GAL3-mbaojin and mYH-UBAP1 with LLOMe 1 mM or 2 mM treatment for 15 min and wash out for 10 min. d 2D-SIM microscopy of COS-7 cells co-transfected GFP-UBAP1^{Wild type} (UBAP1^WT)/GFP-UBAP1^Mutant (UBAP1^Mut) with LAMP1-mYH were treated with mock or LLOMe (1 mM) for 15 min, then washed extensively and chased in the absence of LLOMe for 10 min. e Quantification of UBAP1 puncta on LAMP1 in (d). Mock: UBAP1^WT n = 17, UBAP1^Mut n = 17; LLOMe: UBAP1^WT n = 15, UBAP1^Mut n = 19; Wash out: UBAP1^WT n = 19, UBAP1^Mut n = 19 cells. f Interaction between UBAP1 and LAMP1. COS-7 cells were transfected with UBAP1^WT or UBAP1^Mut and immunoprecipitated with GFP or IgG control antibodies and probed with LAMP1 and VPS37A antibodies. VPS37A was loaded as a positive control. All scale bars: 10 μm. All data represent the mean ± SEM. Two-way ANOVA followed by Sidak’s multiple comparisons test (e), ns p > 0.05, ^#p < 0.0001^. Exact p-values and test statistics are provided as a Source Data file.