Fig. 10: ZIKV-induced neuronal damage and autophagy alterations in the hippocampus of WT and Disc1 KD neonatal mice. | Nature Communications

Fig. 10: ZIKV-induced neuronal damage and autophagy alterations in the hippocampus of WT and Disc1 KD neonatal mice.

From: DISC1 Protects Against Zika Virus Infection and Long-Term Neurological Damage Through AMPK-mTOR-Mediated Autophagy

Fig. 10

a Neonatal mice were intracranially injected with 200 PFU of ZIKV GZ01 or an equal volume of PBS into the λ point of the brain on postnatal day 2 (P2). Mouse brains were collected on D6 post-infection. Representative H&E-stained sections of the hippocampal CA1 region are shown. Black arrows indicate shrunken, hyperchromatic neurons with indistinct boundaries. Orange arrows indicate nuclear pyknosis and fragmentation. Scale bar = 100 μm. b Representative immunofluorescence-stained sections of the hippocampal DG region. Cell nuclei were stained using DAPI (blue). Endogenous Neun was labeled with anti-Neun antibody (red). ZIKV E protein was labeled with anti-E antibody (green). Endogenous LC3A/B was labeled with anti-LC3A/B antibody (yellow). Scale bar = 100 μm. Representative images in (a, b) are from one representative experiment out of two independent experiments with similar results, each including 4 mice per group (n = 4), consisting of 2 males and 2 females.

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