Fig. 8: Shear stress activates GABAB receptor in astrocytes.
From: GABA-independent activation of GABAB receptor by mechanical forces
a Real-time recording of intracellular Ca2+ release in astrocytes under shear stress. Cells were transfected with control siRNA and treated with shear stress, baclofen (100 μM) or ATP (100 μM) in the indicated time point. Data are present as mean ± s.e.m. from 15 cells recorded and are representative from six biologically independent experiments. b Percentage of astrocytes in response to shear stress or baclofen in 236 recorded cells. c Real-time recording of intracellular Ca2+ release in astrocytes under shear stress. Cells are transfected with siRNA knocking down the GABAB receptor and administered to shear stress, baclofen (100 μM), or ATP (100 μM) at the indicated time points. Data are present as mean ± s.e.m. from 45 cells recorded and are representative of six biologically independent experiments. d Percentage of astrocytes with the calcium response (sensitive) or without the calcium response (insensitive) to shear stress (control siRNA: 236 cells; GABAB siRNA: 314 cells). Data are analyzed with χ2 test (two-sided) to determine significance. ****P  <  0.0001.
