Fig. 4: PF exerts anti-inflammatory effects in RAW264.7 cells. | Nature Communications

Fig. 4: PF exerts anti-inflammatory effects in RAW264.7 cells.

From: A mitochondria-targeted fluoropolymer nanoparticle with inherent mitophagy-inducing and red fluorescence properties for treatment of atherosclerosis

Fig. 4

Confocal images (a) and quantitative measurements (b) of ROS in RAW264.7 cells incubated with PF for 6 h. The dose of PF was 32 μg/mL. c Flow cytometry analysis of macrophage subsets in LPS-treated RAW264.7 cells after incubation with the samples for 6 h. Percentage of M2 (iNOS− and CD206+) (d) and M1(iNOS+ and CD206−) (e) macrophages in (c). The concentrations of PF and 5xPF are 32 and 160 μg/mL, respectively. Measurement of IL-6 and TNF-α levels observed upon LPS stimulation with or without treatment with PF/5xPF (f, g). Data are presented as mean ± standard deviation (n = 3 biologically independent samples). Statistically significant difference was analyzed by a two-sided unpaired Student’s t-test. Source data are provided as a Source data file.

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