Fig. 5: plKO male microglia exhibit altered phagocytosis gene expression in the cerebellum.
From: Microglia alter sex-specific cerebellar myelination following placental hormone loss
a Representative gating for FACS of CD11b and pHrodo fluorescence intensity. Thresholds used to categorize High versus Low pHrodo cells are shown. b Percent of pHrodoHigh CD11b+ cells in males and females across development. Data are presented as mean ± s.e.m. Statistical analysis was performed using two-way ANOVAs for sex and genotype, followed by Sidak multiple comparison tests. Male: Control P7 (n = 7), P15 (n = 8), P30 (n = 6); plKO P7 (n = 8), P15 (n = 8), P30 (n = 7). Female: Control P7 (n = 7), P15 (n = 7), P30 (n = 9); plKO P7 (n = 8), P15 (n = 7), P30 (n = 8). ‡‡p < 0.01 for the overall genotype effect (P = 0.0048). c Heatmap representing log2-fold expression changes in a curated list of phagocytosis-related genes determined by high-throughput RT-qPCR on sorted microglia. Statistical comparisons were performed using two-sided, Welch’s multiple t-tests between genotypes at each age. Results are presented as log2-fold change (Log2FC). The number of independent biological replicates for each experiment is listed in the Supplementary Data File. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. d RT-qPCR for Sirpa expression in sorted microglia. Data are presented as mean ± s.e.m. Statistical analysis was performed using two-way ANOVAs for sex and genotype, followed by Sidak multiple comparison tests. Male: Control P7 (n = 7), P15 (n = 8), P30 (n = 6); plKO P7 (n = 8), P15 (n = 8), P30 (n = 7). Female: C P7 (n = 7), P15 (n = 7), P30 (n = 9); plKO P7 (n = 8), P15 (n = 7), P30 (n = 8). ∗∗p < 0.01 for the pairwise comparison (p = 0.0092). Source data are provided as a Source Data file.
