Fig. 7: PGE2 signaling is sex divergent in plKO cerebellum.

a Z-score plot for gene families regulated by Ptger4 at P0, P7, P15, and P30 comparing plKO Male (positive z-score) to plKO Female (negative z-score). b PGE2 levels in the cerebellum at P7, P15 and P30 measured by ELISA. Data are presented as mean concentration ± s.e.m. Statistical analysis was performed using two-way ANOVAs for sex and genotype, followed by Sidak multiple comparison tests. Male: CP7 (n = 7), P15 (n = 8), P30 (n = 8); plKO P7 (n = 8), P15 (n = 8), P30 (n = 6). Female: C P7 (n = 9), P15 (n = 8), P30 (n = 7); plKO P7 (n = 7), P15 (n = 7), P30 (n = 9). #p < 0.05 for the sex by genotype effect (p = 0.0173). c Schematic representation of L-902,688 treatment on cytokine expression at P30 following 6-day injection series of PGE2 agonist, L-902,688 (1 mg/kg/day; P7–P12; in saline; s.c.). Created in BioRender. https://BioRender.com/uto032r. d–h L-902,688 treatment on cytokine expression at P30 following 6-day injection series of PGE2 agonist assessed by multiplex assay. Data are presented as mean ± s.e.m. The number of independent biological replicates for each experiment is listed in the Supplementary Data File. Statistical analysis was performed using two-way ANOVAs comparing sex and genotype, followed by Sidak multiple comparisons tests. ‡p < 0.05, genotype effect; *p < 0.05, **p < 0.01, pairwise comparison. IFN-β1: Male plKO + Veh vs. plKO + L-902,688 (p = 0.0023); Female plKO + Veh vs. plKO + L-902,688 (p = 0.0248). IFNγ: Genotype effect (p = 0.0253). IL-1α: Genotype effect (p = 0.0438). IL-10: Genotype effect (p = 0.0150). M-CSF: Male plKO + Veh vs. plKO + L-902,688 (p = 0.0041); Female plKO + Veh vs. plKO + L-902,688 (p= 0.0033). Source data are provided as a Source Data file.