Fig. 2: Manufactured CAR T cells heterogeneously express CXCR3 and can migrate toward CXCL10. | Nature Communications

Fig. 2: Manufactured CAR T cells heterogeneously express CXCR3 and can migrate toward CXCL10.

From: Engineered CXCR3-A expression enhances B7-H3-targeting CAR T cell migration and efficacy against diffuse intrinsic pontine glioma

Fig. 2: Manufactured CAR T cells heterogeneously express CXCR3 and can migrate toward CXCL10.The alternative text for this image may have been generated using AI.

a CXCR3 expression on unstimulated T cells, anti-CD3/CD28 beads-stimulated T cells, and generated CAR T cells by flow cytometry, representative of n = 2 independent experiments. b Percentage of CAR T cells that migrated from insert wells through 5-µm pores to bottom wells with CXCL10 at the indicated time points. c Relative cell viability of PBT-29FH cells after co-cultured with CXCL10-induced migrated CAR T cells for 24 hours. CAR T cells were seeded on insert wells to migrate through 5-µm pores to bottom wells with CXCL10 for 2 hours before removal of the insert wells. d Relative cell viability of PBT-29FH cells after cultured with CXCL10 for 24 hours. e Relative cell viability of PBT-29FH cells after directly co-cultured with CAR T cells at E:T of 1:1 in the presence of CXCL10 for 24 hours. Cell viability was normalized to the vehicle control without T cells in (c–e). p values were determined by one-way ANOVA with Dunnett’s multiple comparisons test (b, e, n = 3, and c, d, n = 4 independent experiments). Results are presented as means ± SD in (b–e). ns, not significant. Source data are provided as a Source Data file. Illustrations in (b, c) were created in BioRender. Song, E. (2025) https://BioRender.com/bv050hn.

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