Fig. 6: Paracrine chemokines can lead to enhanced efficacy by CXCR3-A-modified CAR T cells against DIPG.

a Relative cell viability of PBT-29FH cells after co-cultured with migrated CAR T cells for 24 hours. CAR T cells were seeded on insert wells to migrate for 2 hours to bottom wells with conditioned medium (CM) from antigen-stimulated CAR T cell culture, before removal of the insert wells. b Relative cell viability of PBT-29FH cells after co-cultured with continuously migrating CAR T cells for 24 hours. CAR T cells were seeded on insert wells and allowed to migrate for 24 hours to the bottom wells without chemoattractant. c Concentrations of CXCL10 by ELISA at the indicated time points in cultures of DIPG cells with added IFN-γ at the indicated doses. p values were determined by randomized block (matching the data for each donor) one-way ANOVA with Dunnett’s multiple comparisons test (a, n = 3 different T cell donors) or paired one-tailed t test (b, n = 3 different T cell donors), or lognormal one-way ANOVA with Dunnett’s multiple comparisons test (c, n = 4 different DIPG models). Results are presented as means ± SD in (a, b). Source data are provided as a Source Data file. Illustration in (b) was created in BioRender. Song, E. (2025) https://BioRender.com/bv050hn.