Fig. 4: Aged host IL-17 promotes conventional IFNγ⁺ T cell trafficking and αPD-L2-driven CD8⁺ T cell polyfunctionality in B16 melanoma.

a–g Flow cytometry data for wild type aged (isotype n = 4 mice, αPD-L2 n = 4 mice), wild type young (isotype n = 5 mice, αPD-L2 n = 4 mice), and IL-17^KO aged mice (isotype n = 5 mice, αPD-L2 n = 4 mice) challenged subcutaneously with B16 melanoma and treated with αPD-L2 (200 µg/mouse) or isotype control (200 µg/mouse) intraperitoneally every 4 days starting on day 3. a Prevalence of tumour-infiltrating CXCR3⁺IFNγ⁺CD8⁺ T cells (CD45⁺CD3⁺B220^-), b CXCR3⁺IFNγ⁺CD4⁺ T cells (CD45⁺CD3⁺B220^-), c Ki67⁺CXCR3⁺IFNγ⁺CD8⁺ T cells (CD45⁺CD3⁺B220^-) d Ki67⁺CXCR3⁺IFNγ⁺CD4⁺ T cells (CD45⁺CD3⁺B220^-) e CCR2⁺CD8⁺ T cells (CD45⁺CD3⁺B220^-) f CCR2⁺CD4⁺ T cells (CD45⁺CD3⁺B220^-). g SPICE-generated graphical pie chart output with overalying phenotype arcs for tumour-infiltrating CD8⁺ T cells. Boolean-gated prevalences within OMIQ were formatted within Pestle and exported into SPICE. Standard error of mean indicated; p-values by (a–f) unpaired Student’s t-test (g) SPICE permutation test.