Fig. 6: Human γδ T cell dynamics in melanomas differ by host age. | Nature Communications

Fig. 6: Human γδ T cell dynamics in melanomas differ by host age.

From: Anti-PD-L2 immunotherapy is efficacious against melanoma in aged hosts through IL-17 and IFNγ signalling

Fig. 6: Human γδ T cell dynamics in melanomas differ by host age.

a–g Data from three pooled human melanoma scRNA-seq datasets (GSE120575 n = 32 human donors, GSE72056 n = 19 human donors, GSE115978 n = 16 human donors). a UMAP projection of γδ T cells stratified into Tγδ1 and Tγδ17 subsets using IFNG and IL17A gene expression, respectively. b Differential gene expression of markers validating the classification of γδ T cells, Tγδ1/Tγδ17 subsets and expression of PDCD1LG2 (PD-L2) in each subset. c Overall prevalence of γδ T cell subsets stratified by age group (young <67 years, old >67 years). d IL17A and IFNG gene expression in Tγδ1/Tγδ17 subsets stratified by patient age. e PDCD1LG2 gene expression in all γδ T cells and Tγδ1/Tγδ17 subsets stratified by patient age. f Statistical analysis of PDCD1LG2 gene expression in all γδ T cells and Tγδ1/Tγδ17 subsets with a median age cutoff of 67 years (young <67 years, old >67 years). g Gene Ontology (GO) analysis of pathways enriched in tumour-infiltrating γδ T cells old versus young patients. p-values by (f) unpaired Student’s t-test (g) GO analysis.

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