Fig. 6: Effects of 5-HT on ABA-induced phosphorylation and stability of ABI5.
From: Engineering hormonal crosstalk to enhance serotonin/melatonin levels in rice
a Measurement of ABI5 phosphorylation levels in rice seedlings treated with ABA alone or in combination with 5-HT (ABA + 5-HT) for 12 h. b Comparison of ABI5 phosphorylation levels between Ubi::T5H transgenic plants and wild-type (WT) following 12 h of ABA treatment. c, d Differential analysis of ABI5 transcript (c) and protein levels (d) in response to treatments with ABA alone and ABA combined with 5-HT. e Evaluation of the impact of phosphorylation on ABI5 protein stability, where ABI5p denotes the phosphorylated ABI5-GST fusion protein co-expressed with SAPK2-His, and ABI5 represents the non-phosphorylated protein expressed independently. f Stability analysis of ABI5 protein in total protein extracts from rice seedlings 12 h post-ABA treatment, compared to untreated controls. In (c), data are presented as means ± SD (n = 3 biological replicates). The experiments depicted in (a, b, d, e, and f) were repeated three times, with consistent results obtained. Source data are provided as the Source Data file.
