Fig. 4: CLCC1 depletion causes formation of nuclear blebs (NBs) in uninfected cells.
From: ER protein CLCC1 promotes nuclear envelope fusion in herpesviral and host processes
a–d TEM images of uninfected cell lines. a single-clone CLCC1-KO (cko3_4), b single-clone CLCC1-KO (cko6_1), c Int_4, d single-clone CLCC1 rescue (cko6_1_R_1). Scale bar = 500 nm. In (a) and (b), NBs are indicated with green arrowheads. Close-up views NBs are shown in insets (c1, c2, d1, and d2), scale bar = 100 nm. Necks connecting some NBs with the INM are indicated with white arrowheads. Images are representative of at least one individual experiments. Additional images are shown in Supplementary Fig. S6. e Quantification of NBs in uninfected cells. Data were combined from two biological replicates. Each dot represents the number of events in a single cell. Bars represent mean values, and the error bars represent SEM. ***: p < 0.001 (p value 0.0001); NS: not significant, p > 0.05 (p values from left to right 0.1653 and >0.9999). Significance was calculated using one-way ANOVA, with multiple comparisons, n = 5 for Int_4 and cko6_1_R_1, n = 4 for cko3_4 and cko6_1. Source data for graphs are provided in Source Data 9.
