Fig. 5: Blocking ACPA-IC binding to monocytes and macrophages with novel antibodies. | Nature Communications

Fig. 5: Blocking ACPA-IC binding to monocytes and macrophages with novel antibodies.

From: Preclinical assessment of two FcγRI-specific antibodies that competitively inhibit immune complex-FcγRI binding to suppress autoimmune responses

Fig. 5

a Gating strategy for monocytes within PBMC fraction, with FSC, SSC, eF780 as a fixable viability dye and CD14-PE to gate for monocytes. b Blocking of mACPA-IC binding to monocytes with the individual compounds. Data from four donors (indicated by square, triangle, circle, and upside-down triangle) at 10µg/ml (closed symbols) or 25µg/ml (open symbols). mACPA-IC measured in quarto and antibody samples in duplo. c Bar graph showing the degree of mACPA-IC binding inhibition to FcγRI on monocytes by C01 (triangle orange), C04 (circle red), or 10.1 (square blue). mACPA-IC only (circle purple) set at 0%. N = 4, in duplo, mean ± SEM, one-way ANOVA with Tukey’s post hoc test. d Gating strategy for monocyte-derived macrophages. e, f similar as b, c but with macrophages, N = 2 donors in duplo, all depicted as mean ± SEM. Source data are provided as a Source Data file.

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