Fig. 1: Nestin⁺ cells are endometrial perivascular cells.

a Immunofluorescence images of P60 CD-1 wild-type uterine longitudinal sections, showing Nestin protein expression specifically in cells adjacent to PECAM1⁺ blood vessels. b–d Immunofluorescence cross-sectional images of P62 Nestin-CreER; Rosa-Tomato uteri exposed to 4-OHT at P60 and P61. Tomato (arrows) was not expressed in ERG⁺ endothelial cells (c) but was expressed in CSPG4⁺ perivascular cells (d). AM, anti-mesometrial pole; M, mesometrial pole; Le, luminal epithelium; Ge, glandular epithelium; S, stroma; Myo, myometrium; Bv, blood vessel. Scale bar: 200 μm. e–g Quantification of percent Nestin⁺ cells expressing Tomato (n = 7 mice) (e), percent Tomato⁺ cells expressing CSPG4 (n = 7 mice) (f), and percent Tomato⁺ cells expressing the epithelial marker KRT8 (n = 7 mice) (g). h Flow cytometry analysis of EpCAM and Tomato expression in P62 Nestin-CreER; Rosa-Tomato uteri exposed to 4-OHT at P60 and P61 (n = 3 mice). i Immunofluorescence images showing expression of CDH1 and Tomato in FACS-purified Tomato^- and Tomato⁺ cells. Scale bar: 100 µm. Data in (e–g, h) are presented as mean ± SD. Source data are provided as a Source Data file.