Fig. 4: Estrogen triggers the differentiation of Nestin+ perivascular cells into epithelial cells via ESR1. | Nature Communications

Fig. 4: Estrogen triggers the differentiation of Nestin+ perivascular cells into epithelial cells via ESR1.

From: Mesenchymal-to-epithelial transition of perivascular cells contributes to endometrial re-epithelialization

Fig. 4

a–d Immunofluorescence images of Nestin-CreER; Rosa-Tomato uteri before mating (a, c) and 6 days after deciduoma induction (b, d) following administration of 4-OHT at P60 and P61. Longitudinal sections were used in (a and c); cross sections were used in (b and d). Percentages of Tomato+ cells expressing ESR1 or PGR are quantified on the right panels of (b) and (d), respectively (n = 3). e Immunofluorescence longitudinal images of Nestin-CreER; Rosa-Tomato uteri after ovariectomy and various hormonal treatments (or oil control). f Quantification of percent Tomato+ epithelial cells (CDH1+) after various hormonal treatments (n = 3, 5, 4, 4, 8 mice from left to right) or oil control. g Immunofluorescence longitudinal images of Nestin-CreER; Rosa-Tomato uteri 6 days and 10 days after deciduoma induction. Percentages of epithelial cells (CDH1+) expressing Tomato are shown on the right in ICI-treated or control oil-treated mice (n = 3 mice). Le luminal epithelium; Ge glandular epithelium; S stroma. Scale bar: 100 μm. Statistical significance was determined using a two-tailed unpaired Student’s t test. *P < 0.05, **P < 0.01. Exact P-values are provided in the Source Data file. Data in (b, d, f, g) are presented as mean ± SD. Source data are provided as a Source Data file.

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