Fig. 6: Transcriptional characterization of cell fate decisions.

a UMAP visualization of original clusters, excluding the acinar population. b Pseudotime values projected on the UMAP primary FoP and CVP dataset. c Schematic overview of cell fate decisions from Lgr5 stem cell towards taste and salivary gland. Created in BioRender. Van de wetering, M. (2025) https://BioRender.com/3ouqtcg. d Schematic overview of cell fate decisions Lgr5 stem cell towards taste. e Trajectory analysis of Lgr5 stem cell towards taste. Feature plot projecting the pseudotime over the subsetted Lgr5 stem cell towards taste dataset. f ‘Gene switches’ reveal top 100 up- and downregulated genes, with several known genes being highlighted (UP:Krt8, Krt7,Scnn1a, Entpd2;Down: Trp63, Krt5, Foxe1). Geneswitch analysis revealing switched-on and -off genes over the pseudotime (x-axis) of cell fate decision towards taste. Surface proteins (green), Transcription Factors(blue), other genes (red). g Schematic overview cell fate decisions Lgr5 stem cell towards saliva. h Trajectory analysis of Lgr5 stem cell towards to salivary glands. Feature plot projecting the pseudotime over the subsetted Lgr5 stem cell towards salivary gland dataset. i ‘Gene switches’ reveal top 100 up- and downregulated genes, with several known genes being highlighted (UP:Krt8, Krt7, Dmbt1;Down: Trp63, Krt5, Sox2). Geneswitch analysis revealing switched-on and -off genes over the pseudotime (x-axis) of cell fate decision towards saliva. Surface proteins (green), Transcription Factors(blue), other genes (red). j Relative expression of TFs (DOWN) over the pseudotime from Lgr5 stem cell to taste lineage (x-axis). k Relative expression of TFs (DOWN) over the pseudotime from Lgr5 stem cell to saliva lineage (x-axis). c, d and g were created with BioRender.com under an academic license.